Journal: iScience

Article Title: The role of long noncoding RNA Nron in atherosclerosis development and plaque stability

doi: 10.1016/j.isci.2022.103978

Figure Lengend Snippet:

Article Snippet: Primary antibodies specific for F4/80 (ab100790, Abcam), α-SMA (ab240654, Abcam) or VEGFA (ab1316, Abcam) was used.

Techniques: Virus, Recombinant, Plasmid Preparation, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Silver Staining, In Vitro, TUNEL Assay, Software

Journal: bioRxiv

Article Title: In vivo expansion of gene-targeted hepatocytes through transient inhibition of an essential gene

doi: 10.1101/2023.07.26.550728

Figure Lengend Snippet: a , Eight-week-old C57BL/6J mice were injected with 5×10 11 GC of AAV-CRISPR and 1×10 11 GC of an AAV-Donor containing the promoterless coding sequences of human FAH (selectable marker) and FIX or 6×10 11 GC of AAV-GFP as control. Mice were injected with Fah -siRNA (3 mg/kg or saline as control) every 4 weeks until 12 weeks post-AAV injection. Blood was collected at time 0 and every two to four weeks and mice were sacrificed at 52 weeks post AAV-injection. Created with BioRender.com. b , Human FIX ELISA in plasma samples. c , Representative immunohistochemistry staining of 2A-tagged FAH (FAH-2A), hematoxylin and eosin (H&E) and Fah in the liver. Scale bar is 100 µm. Data are expressed as mean ± s.d. (n= 3 Control and 9 Unselected and Repair Drive mice) with significance determined by two-way ANOVA followed by Tukey test. **** p<0.0001 Repair Drive vs. Control and Unselected mice.

Article Snippet: Human FIX was measured in 1:16.7 diluted plasma by using Factor IX Human SimpleStep ELISA Kit (ab188393, Abcam), following the manufacturer’s protocol.

Techniques: Injection, CRISPR, Marker, Control, Saline, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Immunohistochemistry, Staining

Journal: Nature cancer

Article Title: Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer

doi: 10.1038/s43018-020-00148-7

Figure Lengend Snippet: Antibodies used for CyCIF

Article Snippet: Error bars represent standard error of the mean (±SEM). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Cycle Channel Antibody Fluorophore Manufacturer Clone Catalog Number Dilution Cycle1 FITC anti-Rabbit-488 Alexa Fluor 488 ThermoFisher Polyclonal A-21246 1:2000 Cy3 anti-Rat-555 Alexa Fluor 555 ThermoFisher Polyclonal A-21434 1:2000 Cy5 anti-Mouse-647 Alexa Fluor 647 ThermoFisher Polyclonal A-21237 1:2000 Cycle 2 FITC anti-GZMB anti-Rabbit-488 Abcam Polyclonal AB4059 1:500 Cy3 anti-GZMB anti-Rat-555 eBioscience 496B 14–8889-80 1:250 Cy5 anti-GZMB anti-Mouse-647 Dako GrB-7 M7235 1:200 Cycle 3 FITC CD8a-488 Alexa Fluor 488 eBioscience AMC908 53–0008-80 1:400 Cy3 CD3D-555 Alexa Fluor 555 Abcam EP4426 {"type":"entrez-nucleotide","attrs":{"text":"AB208514","term_id":"84781018","term_text":"AB208514"}} AB208514 1:200 Cy5 CD45–647 Alexa Fluor 647 BioLegend HI30 304020 1:200 Cycle 4 FITC CD68–488 Alexa Fluor 488 CST D4B9C 24850S 1:200 Cy3 Foxp3–570 eFluor 570 eBioscience 236A/E7 41–4777-82 1:150 Cy5 CD14–647 Alexa Fluor 647 Abcam Polyclonal ab196169 1:400 Cycle 5 FITC CD163–488 Alexa Fluor 488 Abcam {"type":"entrez-protein","attrs":{"text":"EPR14643","term_id":"523380394","term_text":"EPR14643"}} EPR14643 –36 ab218293 1:200 Cy3 Keratin-570 eFluor 570 eBioscience AE1/AE3 41–9003-82 1:400 Cy5 PD1–647 Alexa Fluor 647 Abcam EPR4877(2) ab201825 1:200 Cycle 6 FITC CD4–488 Alexa Fluor 488 R&D Systems Polyclonal FAB8165G 1:200 Cy3 Ki67–570 eFluor 570 eBioscience 20Raj1 41–5699-80 1:200 Cy5 PDL1–647 Alexa Fluor 647 CST E1L3N 15005 1:200 Open in a separate window Antibodies used for CyCIF In BRCA1 -associated TNBC, macrophages were strikingly the most abundant immune cell population in the tumor, as demonstrated by a significant enrichment of CD68 + and CD163 + cells.

Techniques:

Journal: Nature cancer

Article Title: Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer

doi: 10.1038/s43018-020-00148-7

Figure Lengend Snippet: Antibodies used for murine flow cytometry

Article Snippet: Error bars represent standard error of the mean (±SEM). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Cycle Channel Antibody Fluorophore Manufacturer Clone Catalog Number Dilution Cycle1 FITC anti-Rabbit-488 Alexa Fluor 488 ThermoFisher Polyclonal A-21246 1:2000 Cy3 anti-Rat-555 Alexa Fluor 555 ThermoFisher Polyclonal A-21434 1:2000 Cy5 anti-Mouse-647 Alexa Fluor 647 ThermoFisher Polyclonal A-21237 1:2000 Cycle 2 FITC anti-GZMB anti-Rabbit-488 Abcam Polyclonal AB4059 1:500 Cy3 anti-GZMB anti-Rat-555 eBioscience 496B 14–8889-80 1:250 Cy5 anti-GZMB anti-Mouse-647 Dako GrB-7 M7235 1:200 Cycle 3 FITC CD8a-488 Alexa Fluor 488 eBioscience AMC908 53–0008-80 1:400 Cy3 CD3D-555 Alexa Fluor 555 Abcam EP4426 {"type":"entrez-nucleotide","attrs":{"text":"AB208514","term_id":"84781018","term_text":"AB208514"}} AB208514 1:200 Cy5 CD45–647 Alexa Fluor 647 BioLegend HI30 304020 1:200 Cycle 4 FITC CD68–488 Alexa Fluor 488 CST D4B9C 24850S 1:200 Cy3 Foxp3–570 eFluor 570 eBioscience 236A/E7 41–4777-82 1:150 Cy5 CD14–647 Alexa Fluor 647 Abcam Polyclonal ab196169 1:400 Cycle 5 FITC CD163–488 Alexa Fluor 488 Abcam {"type":"entrez-protein","attrs":{"text":"EPR14643","term_id":"523380394","term_text":"EPR14643"}} EPR14643 –36 ab218293 1:200 Cy3 Keratin-570 eFluor 570 eBioscience AE1/AE3 41–9003-82 1:400 Cy5 PD1–647 Alexa Fluor 647 Abcam EPR4877(2) ab201825 1:200 Cycle 6 FITC CD4–488 Alexa Fluor 488 R&D Systems Polyclonal FAB8165G 1:200 Cy3 Ki67–570 eFluor 570 eBioscience 20Raj1 41–5699-80 1:200 Cy5 PDL1–647 Alexa Fluor 647 CST E1L3N 15005 1:200 Open in a separate window Antibodies used for CyCIF In BRCA1 -associated TNBC, macrophages were strikingly the most abundant immune cell population in the tumor, as demonstrated by a significant enrichment of CD68 + and CD163 + cells.

Techniques:

Journal: Nature cancer

Article Title: Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer

doi: 10.1038/s43018-020-00148-7

Figure Lengend Snippet: Antibodies used for human flow cytometry

Article Snippet: Error bars represent standard error of the mean (±SEM). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Cycle Channel Antibody Fluorophore Manufacturer Clone Catalog Number Dilution Cycle1 FITC anti-Rabbit-488 Alexa Fluor 488 ThermoFisher Polyclonal A-21246 1:2000 Cy3 anti-Rat-555 Alexa Fluor 555 ThermoFisher Polyclonal A-21434 1:2000 Cy5 anti-Mouse-647 Alexa Fluor 647 ThermoFisher Polyclonal A-21237 1:2000 Cycle 2 FITC anti-GZMB anti-Rabbit-488 Abcam Polyclonal AB4059 1:500 Cy3 anti-GZMB anti-Rat-555 eBioscience 496B 14–8889-80 1:250 Cy5 anti-GZMB anti-Mouse-647 Dako GrB-7 M7235 1:200 Cycle 3 FITC CD8a-488 Alexa Fluor 488 eBioscience AMC908 53–0008-80 1:400 Cy3 CD3D-555 Alexa Fluor 555 Abcam EP4426 {"type":"entrez-nucleotide","attrs":{"text":"AB208514","term_id":"84781018","term_text":"AB208514"}} AB208514 1:200 Cy5 CD45–647 Alexa Fluor 647 BioLegend HI30 304020 1:200 Cycle 4 FITC CD68–488 Alexa Fluor 488 CST D4B9C 24850S 1:200 Cy3 Foxp3–570 eFluor 570 eBioscience 236A/E7 41–4777-82 1:150 Cy5 CD14–647 Alexa Fluor 647 Abcam Polyclonal ab196169 1:400 Cycle 5 FITC CD163–488 Alexa Fluor 488 Abcam {"type":"entrez-protein","attrs":{"text":"EPR14643","term_id":"523380394","term_text":"EPR14643"}} EPR14643 –36 ab218293 1:200 Cy3 Keratin-570 eFluor 570 eBioscience AE1/AE3 41–9003-82 1:400 Cy5 PD1–647 Alexa Fluor 647 Abcam EPR4877(2) ab201825 1:200 Cycle 6 FITC CD4–488 Alexa Fluor 488 R&D Systems Polyclonal FAB8165G 1:200 Cy3 Ki67–570 eFluor 570 eBioscience 20Raj1 41–5699-80 1:200 Cy5 PDL1–647 Alexa Fluor 647 CST E1L3N 15005 1:200 Open in a separate window Antibodies used for CyCIF In BRCA1 -associated TNBC, macrophages were strikingly the most abundant immune cell population in the tumor, as demonstrated by a significant enrichment of CD68 + and CD163 + cells.

Techniques: